Part:BBa_J100095:Experience

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Applications of BBa_J100095

After plating our cells, specific colonies were placed within specific tubes. The positive control contained the positive control colony including ampicillin. The negative control only contained ampicillin. There are two tubes that contained the plasmid without the inserted promoter, one with maltose and one without. There are also two tubes that contained the ligased plasmid, one with maltose, one without. All these tubes contained ampicillin.

200 microliters of sample from each tube were placed in wells, spacing each sample out; their locations were recorded. There are also two wells that contained the ampicillin as the control, to determine that the fluorescence did not come from the ampicillin. The plates were placed in the synergy machine, in which the fluorescence and the density of each sample were determined.

With the provided data from the synergy machine, the members were able to manipulate the data and create a graph to examine the fluorescence emitted from each sample.

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